Occurrence of fungal microbial contamination in drinking water of megacity of Karachi (Pakistan) and their physico-chemical control.

The water quality in Karachi (Pakistan) is uncertain due to the occurrence of fungi and other microorganisms. A total of twenty-five water samples were collected from public places, educational institutes, hospitals, water supply systems and surface water of the canal of Karachi (Pakistan). The different fungal species including Acremonium sp., Alternaria alternata, Aspergillus flavus, A. fumigatus, A. sulphureus, Cladosporium sp., Fusarium sp., Clonostachys (Gliocladium) sp., Macrophomina phaseolina, Mucor racemosus, Paecilomyces sp. Penicillium chrysogenum, P. citrinum, P. commune, P. expansum, Rhizoctonia sp. and Stachybotrys sp. were isolated from these drinking water samples. However, the bacteria, microalgae and some other microorganisms were present in low concentrations. The reason for fungi infection and production of mycotoxicity depends upon various factors and the availability of their nutrients in filtration plants. The major threats to human health are fungal mycotoxicity which is responsible for carcinogenic and other lethal diseases. Mostly, the genus Aspergillus was dominated and isolated with a maximum of 88-98% of occurrence in the different samples of drinking water by the direct plate-spread method. For the control of fungi, various Physico-chemical coagulation treatments were used, but Potassium alum, clay pot, and hot water treatment disinfected effectively 69-70% removal of the fungi and its spore or mycelia from the water. In addition, it is concluded that drinking water purifications such as chlorination, filtration and lime did not eliminate thermophilic fungal spores or mycelia including Penicillium, Paecilomyces and Mucor from the water.

Energy transition and environmental stability prospects for OECD economies: The prominence role of environmental governance, and economic complexity: Does the geopolitical risk matter?

The global increase in temperature and climate change signals the need for humanity to reduce greenhouse gas emissions and to adopt eco-friendly lifestyles. The 2023 United Nations Climate Change Conference (COP28) in the UAE emphasized this, urging nations to commit to the Paris Agreement and pursue a greener, carbon-free future. In recent decades, climate change has become a critical issue, primarily because of the extensive use of fossil fuels and conventional energy resources. Economic growth has led to an increase in energy consumption and widespread environmental damage. The present study empirically explores whether any changes in environmental governance, economic complexity, geopolitical risk, and the interaction term influence energy transition and environmental stability in OECD economies over the period 1990-2021. Novel econometric methods, including Westerlund co-integration and the Method of Moments Quantile Regression (MMQR), are employed to address complexities such as cross-sectional dependency and panel causality. The key findings from the MMQR technique showed a positive link between environmental governance and economic complexity in driving sustainable energy transitions, thus bolstering environmental resilience in OECD countries. However, economic complexity counterbalances environmental stability. Significantly, geopolitical risk acts as a moderating variable, enhancing the effects of governance and complexity on sustainable energy practices and environmental stability. Based on these insights, this study recommends strategic initiatives, including investment in eco-friendly technologies, to fast-track the shift to clean energy and strengthen environmental resilience in OECD countries. These strategies align with the broader objectives of global sustainable development, offering a path towards a greener and more sustainable future.

Do environmental taxes, environmental innovation, and energy resources matter for environmental sustainability: Evidence of five sustainable economies.

This study explores the relationship between environmental taxation, environmental technologies, energy resources, and consumption-based carbon emissions in five leading green economies from 2000 to 2019. The study applied the Cross-Sectional Auto-Regressive Distributed Lag (CS-ARDL) model to derive benchmark results, with Augmented Mean Group (AMG) and Common Correlated Effect Mean Group (CCEMG) techniques being utilized for conducting robustness analyses. The empirical findings suggest that environmental taxation, environmental innovations, and the consumption of renewable energy are associated with a reduction in consumption-based carbon emissions, thereby contributing to enhanced environmental sustainability. Conversely, the utilization of non-renewable energy is linked to an increase in consumption-based carbon emissions. These results align with the objectives outlined in the Sustainable Development Goals' 2030 agenda, particularly SDG 7 (Affordable and Clean Energy), SDG 9 (Industry, Innovation, and Infrastructure), and SDG 13 (Climate Action), offering valuable policy implications.

Do green logistics and green finance matter for achieving the carbon neutrality goal?

Global warming, a persistent issue, needs comprehensive solutions. Shifting to sustainable resources and promoting green initiatives like green logistics, green investments, and environmental policies (such as environmental technology and environmental tax) are potential ways to address this challenge. The current study explores relationships between these factors and transportation emissions in China, with a focus on achieving the 2060 carbon neutrality goal. To investigate the research gap, the study employs the novel econometric, method of moments quantile regression (MMQR) for benchmark estimation and the bootstrap quantile regression (BSQR) technique for sensitivity estimations from 2000/Q1 to 2019/Q4, and the study confirms the hypothesis of "carbon neutrality." The results reveal that green logistics and green finance have a negative impact on transportation emissions across all quantiles. Environmental technology, environmental tax, and renewable energy also help reduce transportation emissions. On the contrary, economic growth increases transportation emissions, with a greater effect in the early quantile stages but a diminishing impact in later stages. Based on the study's findings, policymakers should prioritize sustainable development strategies to achieve the goal of "carbon neutrality." Implementing green logistics and promoting green investments are essential steps in this direction. Additionally, greater support should be given to the renewable energy sector, green technologies, and sustainable growth to achieve the carbon neutrality goal in China by 2060.

Phylogenetic analysis of promoter regions of human Dolichol kinase (DOLK) and orthologous genes using bioinformatics tools.

The Dolichol kinase (DOLK) gene encodes the polytopic DOLK protein associated with the endoplasmic reticulum (ER) N-glycosylation pathway catalyzing the final step in the biosynthesis of dolichol phosphate. Dolichol phosphate is an oligosaccharide carrier required for N-glycosylation of DOLK protein, with its deficiency leading to a severe hypo glycosylation phenotype in humans which can cause congenital disorders of glycosylation and death in early infancy. The aim of the present study is to identify the phylogenetic relationship between human and ortholog species based on their conserved sequences in DOLK gene. Sequence alignment of DOLK was carried out in this study and the evolutionarily conserved regulatory sequences were identified using bioinformatics. Promoter sequence of human DOLK was compared with orthologous sequences from different organisms. Conserved non-coding sequences (CNS) and motifs in promoter regions were found by analyzing upstream promoter sequences of Homo sapiens DOLK and its orthologous genes in other organisms. Conserved sequences were predicted in the promoter regions in CNS1 and CNS2. Conserved protein sequences were also identified by alignment of the orthologous sequences. Organisms with similar gene sequences are assumed to be closely related and the ER N-glycosylation pathway is conserved in them.

Exploration of plant growth promoting traits and regulatory mechanisms of Bacillus anthracis PM21 in enhancing salt stress tolerance in maize.

Bacillus species have been reported to reduce the negative effects of salt stress on plants; the involvement of Bacillus anthracis PM21 and the internal mechanisms involved in this process are unclear. The effects of PM21 inoculation on maize plants under salt stress were investigated in this study. The study aimed to assess the ability of Bacillus anthracis PM21 to endure high levels of salinity stress while preserving the concentration of plant growth regulators. The biomass, photosynthetic pigments, relative water content (RWC), antioxidants, osmoprotectants, inorganic ion contents, regulation of plant hormones and expression of antioxidants enzyme encoded genes were investigated under normal and salinity stress conditions. Bacillus anthracis PM21 produced a significant amount of 1-aminocyclopropane-1-carboxylate deaminase enzyme (ACC deaminase) and exopolysaccharides (EPS) under salt stress and normal conditions. PM21 also produced plant growth stimulants including indole acetic acid, gibberellic acid (GA3), kinetin, and siderophore under salinity stress and normal conditions. Under salt stress, PM21 inoculation markedly increased plant growth indices, stimulate antioxidant enzyme mechanisms, osmoprotectants, and chlorophyll content. The use of qRT-PCR to analyze the transcription of targeted genes indicated greater expression of antioxidant-encoded genes and inferred their possible function in salinity stress tolerance. Our findings shed light on the functions of PM21 and its regulatory mechanisms in plant salt stress tolerance, as well as the importance of PM21 in this process. This study will provide a thorough analysis of the theoretical framework for adopting PM21 in agricultural production as an eco-friendly method to enhance crop growth and yield under salinity stress.

A cross-cultural study of high-altitude botanical resources among diverse ethnic groups in Kashmir Himalaya, India.

BACKGROUND: In the Himalayas, traditional knowledge and biodiversity are strongly linked due to the symbiotic interaction between plant and cultural diversity, as well as the support provided by cultural memories, ecological awareness, and social norms. Our study was focused on documenting the vanishing knowledge in the Kashmir Himalaya with the following main objectives: 1) to document the ethnomedical and cultural knowledge of the local flora, 2) to evaluate the cross-cultural use of the flora in the region, and, finally, 3) to identify the key indicator species utilized by each ethnic group using multivariate statistical analysis. METHODS: We used semi-structured questionnaires to conduct interviews with people of different ethnicity, gender, age, and occupational categories. The intercultural relationships of species utilization among ethnic groups were examined using a Venn diagram. The overall trends between the indicator values and the plant species used by diverse ethnic groups were illustrated using the linear regression model. RESULTS: We recorded 46 species belonging to 25 different families used by the local people of the Kashmir Valley belonging to four ethnic groups (Gujjar, Bakarwal, Pahari, and Kashmiri). The dominant families recorded were Asteraceae and Ranunculaceae followed by Caprifoliaceae. Rhizomes were the most utilized plant part, followed by leaves. A total of 33 ailments were treated with plants, and gastrointestinal disorders were treated with most species followed by musculoskeletal diseases and dermatological problems. Across cultural relationships, the Gujjar and Pahari showed greater similarity (17%). This may be due to the fact that both ethnic groups share a common geographical landscape and are exogamous to each other. We identified key indicator species used by different ethnic groups with significant (p ≤ 0.05) values. For instance, in the Gujjar ethnic group, Aconitum heterophyllum and Phytolacca acinosa had significant indicator value, which was due to the fact that these plants were easily accessible and also had a wide range of uses. In contrast, the Bakarwal ethnic group showed different indicator species, with Rheum spiciforme and Rhododendron campanulatum being highly significant (p ≤ 0.05), because this ethnic group spends the majority of their time in high-altitude pastures, using a particularly wide variety of plant species for medicine, food, and fuelwood. While indicator values and plant usage were positively correlated for the Gujjar, Kashmiri, and Pahari ethnic groups, they were negatively correlated for the Bakarwal. The positive correlation indicates cultural preferences for certain plant use and underlines the cultural significance of each species. The current study reported new uses for the following species: raw roots of Jurinea dolomiaea used for tooth cleaning, seeds of Verbascum thapsus applied for respiratory diseases, and flowers of Saussurea simpsoniana given to anyone as a good luck wish. CONCLUSION: The current study highlights historical ethnic group stratifications and cultural standing while comparing reported taxa across cultures. Each ethnic group made extensive ethnomedical use of plants, and knowledge, originally transmitted verbally, is now available in writing for reference. This could pave the way for providing incentives to local communities to showcase their talents, celebrate them, and gain from potential development initiatives.

Antifungal activity of green synthesized selenium nanoparticles and their effect on physiological, biochemical, and antioxidant defense system of mango under mango malformation disease.

Plant extract-based green synthesis of nanoparticles is an emerging class of nanotechnology that has revolutionized the entire field of biological sciences. Green synthesized nanoparticles are used as super-growth promoters and antifungal agents. In this study, selenium nanoparticles (SeNPs) were synthesized using Melia azedarach leaves extract as the main reducing and stabilizing agent and characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), energy-dispersive X-ray (EDX), and fourier transform infrared spectrometer (FTIR). The green synthesized SeNPs were exogenously applied on Mangifera indica infected with mango malformation disease. The SeNPs at a concentration of 30 µg/mL were found to be the best concentration which enhanced the physiological (chlorophyll and membrane stability index), and biochemical (proline and soluble sugar) parameters. The antioxidant defense system was also explored, and it was reported that green synthesized SeNPs significantly reduced the biotic stress by enhancing enzymatic and non-enzymatic activities. In vitro antifungal activity of SeNPs reported that 300 µg/mL concentration inhibited the Fusarium mangiferae the most. This study is considered the first biocompatible approach to evaluate the potential of green synthesized SeNPs to improve the health of mango malformation-infected plants and effective management strategy to inhibit the growth of F. mangifera.

Species distribution modelling of Monotheca buxifolia (Falc.) A. DC.: Present distribution and impacts of potential climate change.

Species distribution modelling (SDM) is an important tool to examine the possible change in the population range and/or niche-shift under current environment and predicted climate change. Monotheca buxifolia is an economically and ecologically important tree species inhabiting Pakistan and Afghanistan in dense patches, and species range is contracting rapidly. This study hypothesize that predicted climate change might remarkably influence the existing distribution pattern of M. buxifolia in the study area. A total of 75 occurrence locations were identified comprising M. buxifolia as a dominant tree species. The Maximum Entropy (MaxEnt) algorithm was utilized to perform the SDM under current (the 1970s-2000s) and two future climate change scenarios (shared socioeconomic pathways: SSPs 245 and 585) of two time periods (the 2050s and 2070s). The optimal model settings were assessed, and simulation precision was assessed by examining the partial area under the receiver operating characteristic curve (pAUC-ROC). The results showed that out of 39 considered bio-climatic, topographic, edaphic, and remote sensing variables which were utilized in the preliminary model, 6 variables including precipitation of warmest quarter, topographic diversity, global human modification of terrestrial land, normalized difference vegetation index, isothermality, and elevation (in order) were the most influential drivers, and utilized in all reduced SDMs. A high predictive performance (pAUC-ROC; >0.9) of all the considered SDMs was recorded. A total of about 67,684 km2 of geographical area was predicted as suitable habitat (p > 0.8) for M. buxifolia, and Pakistan is the leading country (with about 54,975 km2 of suitable land area) under the current climate scenario. Overall, the existing distribution of the tree species in the study area might face considerable loss (i.e. rate of change %; -27 to -107) in future, and simultaneously a northward (high elevation) niche shift is predicted for all the considered future climate change scenarios. Hence, development and implementation of a coordinated conservation program is required on priority basis to save the tree species in its native geographic range.

Effect of transcranial magnetic stimulation on postural control of individuals with major depressive disorder: A case report.

Accidental falls and major depressive disorder (MDD) are two common conditions associated with aging. Initial treatment of MDD often starts with administering antidepressants, followed by transcranial magnetic stimulation (TMS) for treatment-resistant individuals. The purpose of this case study was to determine the effect of repetitive TMS (rTMS) on postural control of an individual with MDD. A 44-year-old male with recurrent severe MDD was assessed for postural balance during eyes closed and eyes open conditions, pre and post three consecutive sessions receiving high-frequency rTMS (NeuroStar). Total excursion and velocity of sway significantly decreased following rTMS treatment when eyes were closed (p < 0.05). Power of the sway changed, but the changes were not statistically significant. The fractal dimension confidence circle area decreased significantly in eyes closed trials (p < 0.05). It appears that rTMS application can potentially impact postural steadiness in individuals with MDD. Our results warrant further studies with larger study samples.

Endorsing sustainable development in BRICS: The role of technological innovation, renewable energy consumption, and natural resources in limiting carbon emission.

This research aimed to examine the complex interaction between technological innovation, renewable energy consumption, natural resources, and carbon dioxide (CO2) emissions of BRICS (i.e., Brazil, Russia, India, China, and South Africa) countries from 1990 to 2019, to accomplish the Paris Climate Conference (COP-21) objective of reducing CO2 emissions to promote environmental sustainability. The long-run empirical estimations derived from the CS-ARDL technique, which considered other estimation issues like cross-sectional dependency and slope heterogeneity, indicated that technological innovation, renewable energy consumption, and natural resources increase environmental sustainability by limiting CO2 emissions, in the short-run and long-run. The technological innovation-related activities have a CO2 mitigating effect as shown by the negative coefficients which ranges between -0.05 and -0.14. This shows that they increase environmental sustainability and aid in achieving Sustainable Development Goals (SDGs) 13. Similarly, renewable energy and natural resources decrease CO2 emissions as shown by the coefficient of renewable energy (-0.31 to -0.81) and natural resources (-0.01 to 0.95); thereby increasing ecological quality by limiting CO2 emissions. Furthermore, the interaction of technological innovation with natural resource rent and renewable energy consumption also aids in mitigating CO2 emissions and increases environmental health. Finally, panel causality analysis revealed a significant causality from all explanatory variables to CO2 emissions. Based on the results, significant policy suggestions are provided, such as improving energy effectiveness, investing in energy technologies, and increasing renewable energy consumption to stimulate technological innovation to achieve the target of a net-zero­carbon economy.

Assessment of variability in nutritional quality of wild edible fruit of Monotheca buxifolia (Falc.) A. DC. Along the altitudinal gradient in Pakistan.

The fruit of Monotheca buxifolia is among the underutilized wild edible fruits that grows in Hindukush and Suleiman range mountains of Pakistan. Mountain communities consume this wild fruit as a food, medicine and it provides an important source of income. In this study, we aimed to investigate the total yield and the effect that altitude plays on its proximate composition and mineral contents as determined through phytochemical screening of this economically important wild fruit. Our results indicate a significant increase in the total fruit yield with increasing trunk diameter (R2 = 0.98), height (R2 = 0.95) and cover (R2 = 0.92). The proximate composition shows that the crude fat and carbohydrate contents of Monotheca fruit significantly varies (P < 0.05) along the altitudinal gradient. Similarly, ANOVA followed by post hoc Tukey HSD, further confirms the variation (P < 0.01) in moisture contents of the fruit. Dry matter ranged from 95.28 ± 4.64 to 108 ± 3.70 g kg- 1 of the fruit's edible portion, while protein contents varied between 17.16 % and 20.44 %. The fruits were found to be rich in minerals containing sizeable amounts of potassium, iron, phosphorus, sodium, nitrogen, magnesium, and copper. Significant difference was observed in the nitrogen (P < 0.01), potassium (P < 0.05) and sodium (P < 0.05) contents of the fruit along the altitudinal gradient. Similarly, tannins, flavonoids, saponins, reducing sugars, terpenoids, anthraquinones, and cardiac glycosides, were the most prominent chemicals found in Monotheca fruit. It was concluded that Monotheca fruit is a good source of carbohydrates, proteins, macro and micro-nutrients which fluctuated along the altitudinal gradient.

Biochemical Characterization of Halotolerant Bacillus safensis PM22 and Its Potential to Enhance Growth of Maize under Salinity Stress.

Salinity stress is one of the primary abiotic stresses limiting crop growth and yield. Plants respond to salinity stress with several morphophysiological, molecular, and biochemical mechanisms, however, these mechanisms need to be improved further to cope with salt stress effectively. In this regard, the use of plant growth-promoting (PGP) and halotolerant bacteria is thought to be very efficient for enhancing growth and salinity tolerance in plants. The current study aims to assess Bacillus safensis PM22 for its ability to promote plant growth and resistance to salt. The PM22 produced substantial amounts of exopolysaccharides, indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylic acid deaminase (ACC-deaminase) under saline conditions. Additionally, inoculation of the halotolerant bacteria PM22 reduced the severity of salinity stress in plants and increased root and shoot length at various salt concentrations (0, 180, 240, and 300 mM). Furthermore, PM22-inoculated plants showed markedly enhanced photosynthetic pigment, carotenoid, leaf relative water content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity, salt tolerance index, total soluble sugar, total protein, and ascorbic acid contents compared to non-inoculated control maize plants. PM22 substantially increased antioxidant (enzymatic and non-enzymatic) activities in maize plants, including ascorbate peroxidase, peroxidase, superoxide dismutase, catalase, total flavonoid, and phenol levels. Maize plants inoculated with PM22 also exhibited a significant reduction in electrolyte leakage, hydrogen peroxide, malondialdehyde, glycine betaine, and proline contents compared to non-inoculated control plants. These physiological appearances were further validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), which revealed the upregulation of expression in genes responsible for stress tolerance. In the current investigation, Bacillus safensis PM22 showed plant growth-promoting and salt tolerance attributes and can be utilized as a bio-inoculant to improve yield in salt stress affected areas.

The nexus of financial development, technological innovation, institutional quality, and environmental quality: evidence from OECD economies.

The present study investigates the effect of institution quality, technological innovation, and financial development on environment quality using 37 OECD nations from 1998 to 2018. The cross-sectional dependence (CD) and Lagrange multiplier (LM) techniques are used to measure the cross-sectional dependence. The second-generation panel unit root tests and panel cointegration tests are applied to examine the unit-root properties and long-run association existence between variables. Finally, we employed the two-step (SYS-GMM) methodology to estimate the coefficient values. The findings showed that financial development has a positive effect on selected carbon (CO2) emission dimensions. When the moderating term is introduced, it was identified that institutional quality and technology innovation conditioning effects are crucial between financial development and CO2 emission. Our evidence-based study provides significant results for technology innovation and institutional quality moderating role in reducing CO2 emissions in OECD economies. Our findings are also robust to alternative measures, which could be useful for policymakers to formulate long-term and short-term strategies and policies for a better sustainable environment.

Environmental concerns of financial inclusion and economic policy uncertainty in the era of globalization: evidence from low & high globalized OECD economies.

Environmental consequences of financial aspects, policy uncertainties and rapid globalization is the topic of intense debate in present years. However, this study contribute to existing literature in an innovative way. We classified the 33 OECD economies in two group's lower globalized economies (LGE) and highly globalized economies (HGE), based on their level of globalization. Considering the cross-sectional dependency and slope heterogeneity in the data this study employed the Augmented Mean Group method to estimate the influence of financial inclusion, economic policy uncertainty and globalization on the environment quality of both groups for the period 1996-2019. The results revealed a negative significant impact of financial inclusion, while a positive significant impact of economic policy uncertainty on CO2 emissions in both groups, LGE and HGE. However the globalization estimated to have positive impact on CO2 emission in LGE's, in HGE's it is significantly impeding the CO2 emission. The interaction of globalization with financial inclusion and economic policy uncertainty respectively found negative and positive to effect the CO2 in both LGE's and HGE's. The study suggests that, LGE's are need to prepare for economic globalization, move toward adopting energy-efficient technology and promote trade in less-polluting products in order to sustain their environment quality. The outcomes of this study are robust by employing different model specifications.

Which factor explains the life-history of Xanthium strumarium L., an aggressive alien invasive plant species, along its altitudinal gradient?

Invasive biology acknowledges the concept of better performance by invasive plants in the introduced range. Xanthium strumarium L. is one of the successful invasive species in Khyber Pakhtunkhwa, Pakistan. The phenological pattern, vegetative and reproductive traits plasticity analysis of the species was explored to explain the invasive success across the altitudinal gradient in the current invaded habitats. Phenological patterns and timing (seedling, vegetative growth, flowering and fruiting, drying, and seed bank) were observed during a full year for two seasons. We also examine plant functional traits at altitudes of 500, 1000, and 1500 m a.s.l. to assess traits and biomass variations. The X. strumarium exhibits late vegetative and reproductive phenology at higher altitudes, enabling them to occupy an empty niche and benefit from decreased competition for resource acquisition. The lower altitude plants show a higher growth rate (stem size increase, number of leaves, and leaf area) due to the higher nutrient availability. Higher altitude plants have the highest reproductive biomass and biomass ratio revealing plant abilities to be reproductively adapted in the higher altitudes. Among climatic variables, mean yearly temperature, mean annual yearly humidity, and mean day length in hours, while in soil variables, organic matter and nitrogen percentage significantly affect the phenological and morphological stages. Therefore, we conclude that X. strumarium can invade higher altitudes with a shift in its phenological and morphological changes making the invasion process successful.

Industrial growth and CO2 emissions in Vietnam: the key role of financial development and fossil fuel consumption.

The increase in greenhouse gas emission has a major global issue, catching the attention of the researcher and policymakers around the world. The combustion of fossil fuel is the main cause of the rising greenhouse gas emission particularly in developing countries including Vietnam. Meanwhile, the purpose of the study is to evaluate the linkage between fossil fuel consumption, financial development, industrial progression, and CO2 emission over the period from 1970 to 2019, particularly in Vietnam. The study applied the ARDL econometric technique and Bayer-Hanck cointegration approach with structural break to confirm long run relationship and the EKC hypothesis between industrial growth and CO2 emission, in Vietnam, which assume the U-shaped link between CO2 emission and industrial growth in Vietnam. Which further confirmed by Lind and Mehlum U test in addition, the Granger causality exists between fossil fuel consumption and CO2 emission in both short run and long run. The causal relationship is unidirectional in the short run running from fossil fuel consumption and carbon dioxide emission; therefore, the study proposed to adopt low-carbon emission technology.

Posterior Aneurysmorrhaphy & Lateralization Of Aneurysmal Fistula Vein: A Modification Of Existing Aneurysmectomy Techniques.

BACKGROUND: To present a new modification of aneurysmectomy technique for the management of aneurysmal fistula vein. This cross-sectional study was carried out at Vascular Surgery department, Combined Military Hospital Rawalpindi from 1st October 2014 to 30th December 2018. METHODS: This modified aneurysmectomy procedure was performed in 20 patients. Massive diffuse venous dilatation of vein, necrosis of overlying skin, reduction in the cannulation area and pain were the indications of surgery. Revision was performed by rotating the mobilized vein to 180°, excising redundant vein, reducing diameter, repairing venotomy posteriorly and placing the vein in a mobilized skin flap over the fascia of biceps muscle away from skin suture line, to facilitate subsequent cannulation and post puncture haemostasis. The rationale of this modification was to prevent damage to the usual anteriorly placed suture line during needle insertion for haemodialysis. The data were analysed by using SPSS version 25.0. RESULTS: The mean age of patients was 45.45±14.75 years with a range of 12-65 years. Sixteen patients (80.0%) were having brachiocephalic fistula while 4 patients (20.0%) had radio cephalic fistula. The mean time of reuse of AVF after vein Aneurysmectomy was 24.55±5.3 days with half of the patients having reuse of AVF after 3 weeks. Three (15%) patients developed hematoma while 2 (10%) patients developed skin necrosis. Fifteen (75%) patients had no surgery related complications after procedure. CONCLUSIONS: Posterior suturing and lateralization of aneurysmal vein under mobilized skin flap is a safe, effective and easy to learn modification of aneurysmectomy procedure for the management of aneurysmal dilatation of fistula vein.

Outcome Of Embolectomy In Patients Presenting Late With Acute Limb Ischemia.

BACKGROUND: UThe aim of this study is to share our experience regarding outcome of embolectomy in the patients presenting late with acute limb ischemia. It was a cross sectional descriptive study, conducted at CMH Rawalpindi from January 2016 to December 2017. METHODS: All those patients having non-traumatic acute limb ischemia presenting between 6 to 72 hours of the onset of symptoms with viable affected limb were included. Their data was analysed for demography, involved limb, time of embolectomy, fasciotomy, re embolectomy, amputations, reperfusion injuries and death. The patients presenting later than 72 hours, irreversible Ischemia and limb ischemia secondary to trauma or graft occlusion of previous Bypass surgery were excluded from the study.. RESULTS: A total of 49 (36 males and 13 females) patients were included in the study. Thirty-three (67.3%) patients underwent embolectomy for lower while 16 (32.6%) for upper limb ischemia. Ten (20.4%) patients had fasciotomy while 7 (14.2%) patients underwent re embolectomy. In 5 (10.2%) patients vascular bypass had to be done. Seven (14.2%) patients underwent minor amputations while 5 (10.2%) had major limb amputations. Two (4%) patients died of reperfusion injury. Overall 42 (85.7%) limbs were salvaged. CONCLUSIONS: Embolectomy is effective in late presenting acute limb ischemia with viable extremity and should be offered to these patients.

Starch accumulation in rice grains subjected to drought during grain filling stage.

Drought stress during the grain filling stage severely affects the quality and quantity of starch in rice grains. The enzymes such as ADP-glucose pyrophosphorylase (AGPase, EC 2.7.7.27) and starch synthase (SS, EC 2.4.1.21) are the key regulatory enzymes involved in the starch biosynthesis. In this study, the activity of the AGPase and starch synthase (SS) was correlated with the qualitative and quantitative parameters such as sucrose, starch, amylose, amylopectin, and resistant starch in leaves, roots, and grains of drought tolerant (N22) and drought susceptible (IR64) cultivars under applied water deficit stress (WDS). Drought stress enhanced the remobilization of stored starch from leaves to developing rice grains which was positively correlated with a decrease in the starch and starch synthase activity in leaves. Starch accumulation in developing grains was positively correlated with an increase in the AGPase and SS activity under drought. It was found that starch, amylopectin, and sucrose content in developing grains increased under water deficit stress (WDS), while amylose content decreased in both the varieties. However, in leaves, the SS activity decreased while AGPase activity was found to be increased under WDS in both varieties. Decreased starch content in matured grains was due to shortening of grain filling stage as drought stress caused early plant senescence. Yield reduction under drought was more in susceptible variety IR64 as compared to tolerant genotype N22.